"We demonstrated the applicability of the device to real-world samples through determination of MNPs released from plastic cups after hot water and flow induction and nanoplastics in tap water. Moreover, the device is user-friendly and operative by untrained personnel to conduct data processing on the APP remotely. The analytical platform integrating quantitative imaging, customized data processing, decision tree model, and low-cost analysis ($0.015 per assay) has great potential for high-throughput screening of MNPs in agrifood and environmental systems."