I'll start off by listing mine:
The T-800, Terminator 2: "I know now why you cry, but it's something that I can never do." Gets me every damn time.
ADA, Zone of the Enders: I think I'm the only one who played this game more than the MGS2 demo that came with it. I will never not laugh at the exchange of "You may speak like a human, but you're still a heartless computer, aren't you?" "That is correct. What is the problem?"
Codsworth, Fallout 4: He survives the nuclear holocaust despite not having a bunker and waits 200 years for you to come back. When you look at what changes his relationship with you, he mostly just wants you to be nice to people. I never swapped him out as my companion.
B.O.Y.D., Ducktales 2017: He's adorable. 'nuff said.
That's the fucked thing; I know bazingas that got the take of "see? This is why we need friendly AI so the waifubot is sapient enough to suffer but just keeps taking the abuse forever and ever!"
As far as I'm concerned Ava is entirely correctly aligned.
Me trying to explain to bazingas that publicly traded corporations are real existing paperclip maximizers.