bringing up hologra, even though Choco is allowed be be more mature there as on her own channel, it's usually to highlight the contrast between her adultness and the other girls' seisoness. Not even always sexual, like the one where Watame tries to drink coffee and keeps spitting it out on Subaru. Then they use A-chan for a more relaxed maturity. Or I guess there's also the Matsuri line, where no one can say something worse than Matsuri has already said, and they need to end the stream if they do. Noel also dips into some sexual content, but she stopped her last stream cause she saw the joke going too far.