In June 1810 news arrived from Buenos Aires that Napoleon Bonaparte's forces had conquered Andalusia and laid siege to Cádiz, the last redoubt against the French on Spanish soil. Moreover, the Supreme Central Junta, which had governed the Empire for the past two years, had abolished itself in favor of a Regency Council. García Carrasco, who was a supporter of the carlotist group, managed to magnify the political problems by taking arbitrary and harsh measures, such as the arrest and deportation to Lima without due process of well-known and socially prominent citizens under simple suspicions of having been sympathetic to the junta idea. Among those arrested were José Antonio de Rojas, Juan Antonio Ovalle and Bernardo de Vera y Pintado.

Inspired by the May Revolution in Argentina, the autonomy movement had also propagated through the criollo elite. They resented the illegal arrests and, together with the news that Cádiz was all that was left of a free Spain, finally solidified in their opposition to the Governor. Brigadier García Carrasco was suspended from office and forced to resign on July 16, 1810, to be in turn replaced by the next most senior soldier, Mateo de Toro Zambrano Count of la Conquista, even though a legitimate Governor, Francisco Javier de Elío, had already been appointed by the Viceroy of Peru.

Count Toro Zambrano was, by all standards, a very unorthodox selection. He was a very old man already (82 years old at the time) and moreover a "criollo" (someone born in the colonies) as opposed to a "peninsular" (someone born in Spain). Immediately after his appointment in July, the juntistas began to lobby him in order to obtain the formation of a junta. In August the Royal Appeals Court (Spanish: Real Audiencia) took a public loyalty oath to the Regency Council in front of a massive audience, which put added pressure on the Governor to define himself. After vacillating for some time over which party to follow, Toro Zambrano finally agreed to hold an open Cabildo (city hall) meeting in Santiago to discuss the issue. The date was set for September 18, 1810 at 11 AM.

From the very beginning the juntistas took the political initiative. They were able to place their members in charge of sending the invitations, thus manipulating the assistance lists to their own advantage. At the September 18th session, they grabbed center stage with shouts of "¡Junta queremos! ¡junta queremos!" ("We want a junta! We want a junta"). Count Toro Zambrano, faced with this very public show of force, acceded to their demands by depositing his ceremonial baton on top of the main table and saying "Here is the baton, take it and rule". The discussion ended with the establishment of the Government Junta of the Kingdom of Chile, also known as the First Junta, which was organized with the same powers held by a Royal Governor. In the discussion triumphed the idea of a local independent government as opposed to the monarchist idea of submitting themselves to the control of the Regency Council. Nonetheless, this idea of "independence" must not be taken in the modern sense. This junta never intended to sever the relationship with the monarchy, but was only taking advantage of the possibility to assert local rule.

Thus was established the Government Assembly of the Kingdom of Chile, also known as the First Government Junta. It was the earliest step in the Chilean struggle for independence, and the anniversary of its establishment is celebrated as the national day of Chile. In 1971, President Salvador Allende asked that the celebration become more ecumenical, encompassing the diverse religious beliefs throughout the country.

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