No it was never a bit, but on a semi-recent episode they explained that they had received some feedback from someone who had said something like "woah I recently noticed that the other hosts actually referred to Alice as 'she' in the episode and realized she's actually trans, I thought that was just a bit." And then Alice made some comment like she hadn't done as much work as she could have on her voice but eh, it was a lot of work. They've referred to it as "not a bit" subsequently.