Off the top of my head, SpongeBob SquarePants, Danny Phantom, Fairly Oddparents, The Big Bang Theory and It's Always Sunny in Philadelphia all have jokes, if not the main plot point of the episode, that are just mocking the existence of LGBTQ people.
That episode where timmy wished he was a girl tho made me wish I was a girl
I've actually thought about that episode a decent amount since it came out and I still have no idea if it was (positively) transgressive, if it just reified gender norms, or some weird combination of the two.
Because, like, to some degree they make it about "women be crazy" kind of shit. But there's also that running throughline of people feeling like they can't do things that aren't "for" their gender and how shitty that is