Off the top of my head, SpongeBob SquarePants, Danny Phantom, Fairly Oddparents, The Big Bang Theory and It's Always Sunny in Philadelphia all have jokes, if not the main plot point of the episode, that are just mocking the existence of LGBTQ people.
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That was definitely the intent -- "this is what the lowest form of humor looks like." In the mid-00s, though, that lowest form of humor was dudes calling each other slurs like that.