Off the top of my head, SpongeBob SquarePants, Danny Phantom, Fairly Oddparents, The Big Bang Theory and It's Always Sunny in Philadelphia all have jokes, if not the main plot point of the episode, that are just mocking the existence of LGBTQ people.
I would say that the early episodes had the idea that a male person identifying as a woman was ridiculous.
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The actors have always been pretty trans positive IRL. They also are pretty aware of the fact that neo-nazis really like them because they're morons who don't understand sarcasm and love the fact that they're all white. Which I think is why they did so many "we should kill Nazis" episodes.
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